Dive into Imaging Science
This is the Podcast of the Europen Society for Molecular Imaging (ESMI).
Pour yourself a nice glass of wine and join us as we delve into the recent literature, and perhaps learn a little from the minds that have shaped the field.
The premise is really simple: Each time we invite one senior academic to discuss their favourite publication from the recent literature. We’ll find out what caught their eye, and why it is worth taking a closer look at this particular publication. Along the way, we will learn their publication strategies and possibly even glimpse into the future of this amazing field of research.
Music “In this Life”, Composer: Guido Jäger, Musicians: Andreas Jacobs – violin, Jacob Niller – accordion, Wolfgang Platen – contrabass
Dive into Imaging Science
Multiplexed bioluminescence imaging with a substrate unmixing platform
GUEST: Clemens C.W.G. Lowik, Rotterdam
In this episode we discuss and learn about Bioluminescence Imaging and modern tools for multiplexed imaging of different colors, tackling a major challenge of the field. Our guest is one of the founding fathers of the ESMI and pioneers on multicolor BLI, the one and only Clemens Lowik.
We talk about innovative approaches for unmixing multiple luciferase-luciferin pairs and quantitative analyses of bioluminescent mixtures, enabling serial tracking of heterogeneous cell populations. On the course of our discussion we learn on how such methods will help advance BLI to cover new exciting regimes, but also spill over to the wider optical imaging.
If you’re on to exciting developments of the field - listen carefully!
Selected Publication: "Multiplexed bioluminescence imaging with a substrate unmixing platform" published in Cell Chemical Biology by the authors C. K. Brennan, Z. Yao, A.A. Ionkina, C.M. Rathbun, B. Sathishkumar, J.A. Prescher (University of California, Irvine, USA).
Publication reference: C. K. Brennan, Z. Yao, A.A. Ionkina, C.M. Rathbun, B. Sathishkumar, J.A. Prescher. Multiplexed bioluminescence imaging with a substrate unmixing platform. Cell Chem Biol. 2022 Nov 17;29(11):1649-1660.e4. doi: 10.1016/j.chembiol.2022.10.004. Epub 2022 Oct 24 .
https://www.cell.com/cell-chemical-biology/fulltext/S2451-9456(22)00358-0
Further information on the European Society for Molecular Imaging:
https://e-smi.eu/
Contact: office@e-smi.eu
